Latex C-serum from Hevea brasiliensis induces non-apoptotic cell death in hepatocellular carcinoma cell line (HepG2)
Article first published online: 10 OCT 2012
© 2012 Blackwell Publishing Ltd
Volume 45, Issue 6, pages 577–585, December 2012
How to Cite
Lam, K. L., Yang, K. L., Sunderasan, E. and Ong, M. T. (2012), Latex C-serum from Hevea brasiliensis induces non-apoptotic cell death in hepatocellular carcinoma cell line (HepG2). Cell Proliferation, 45: 577–585. doi: 10.1111/j.1365-2184.2012.00841.x
- Issue published online: 29 OCT 2012
- Article first published online: 10 OCT 2012
- Manuscript Accepted: 5 JUL 2012
- Manuscript Received: 19 FEB 2012
- Malaysian Rubber Board
- Universiti Sains Malaysia. Grant Number: 1001/CIPPM/822111
- Fellowship and Postgraduate Research University Research. Grant Numbers: 1001/CIPPM/843075, 1001/CDADAH/843073
Latex from Hevea brasiliensis (natural rubber tree primarily cultivated for its rubber particles) has no known primary metabolic function, although its biological role is as a plant defence system. The present study has evaluated specific anti-proliferative effects of latex whole C-serum and its subfractions, on human cancer cell lines.
Materials and methods
Cell viability assay using MTT, DNA fragmentation assay and real-time PCR were used to evaluate the cytotoxic effects of latex whole C-serum and its subfractions on the cell lines.
MTT assay revealed very low LC50 values, 2.0 and 280 ng/ml, for DCS and DCP treatments, respectively. DCS was proven to be more potent compared to DCP, in conferring specific anti-proliferative effects on the cancer cell lines. The study also indicated that anti-proliferative activity of pre-heated C-serum fractions diminished significantly.
Although noteworthy cell death was reported, DNA fragmentation assay and real-time PCR confirmed that that induced by latex C-serum subfractions was not promoted via the classical apoptotic signalling pathway.