Basophil leucocyte separation from human peripheral blood: a technique for their isolation in high purity and high yield


Dr R. P. Day, Department of Clinical Immunology, Institute of Diseases of the Chest, Fulham Road, London, S.W.3.


A method is described whereby viable basophil leucocytes are isolated from human peripheral blood by density gradient centrifugation. The rate of recovery averaged 74% and the purity averaged 93% of the final preparation.