The inhibitory effect of serum factors on measurement of IgE aspergillus antibodies by RAST

Authors

  • DANIEL VERVLOET,

    1. Allergy Research Laboratory of the Buffalo General Hospital and the Departments of Medicine and Microbiology of the State University of New York at Buffalo
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  • YOSUKE FUJITA,

    1. Allergy Research Laboratory of the Buffalo General Hospital and the Departments of Medicine and Microbiology of the State University of New York at Buffalo
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  • JOHN I. WYPYCH,

    1. Allergy Research Laboratory of the Buffalo General Hospital and the Departments of Medicine and Microbiology of the State University of New York at Buffalo
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  • ROBERT E. REISMAN,

    1. Allergy Research Laboratory of the Buffalo General Hospital and the Departments of Medicine and Microbiology of the State University of New York at Buffalo
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  • CARL E. ARBESMAN

    Corresponding author
    1. Allergy Research Laboratory of the Buffalo General Hospital and the Departments of Medicine and Microbiology of the State University of New York at Buffalo
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Carl E. Arbesman, M.D., Allergy Research Laboratory, Buffalo General Hospital, 100 High Street, Buffalo, New York 14203, U.S.A.

Summary

The RAST value obtained in the A. fumigalus system, from sera of patients with allergic bronchopulmonary aspergillosis (ABPA) is dependent not only on the reagin titre but also on blocking factors that depress the RAST score. These factors are primarily specific for A. fumigatus and can be removed by an A. fumigatus immuno-adsorbent. However, some blocking activity could be found in normal sera even after absorption with an A, fumigates immunoadsorbent and seems to be non-specific. All of these blocking factors influence the specificity of the RAST, and complicate the comparison of the quantitation of IgE antibodies in different sera. The use of discs rather than cellulose particles and the use of dilute sera result in a RAST assay more proportional to the specific IgE antibodies to A. fumigates.

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