Clinical & Experimental Allergy

Mites and house dust allergy. IV. Antigen and allergen(s) of Dermatophagoides pteronyssinus extract

Authors

  • G. BILIOTTI,

    1. Clinical Immunology Service, Institute of Medical Pathology, University of Florence, Florence
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  • S. ROMAGNANI,

    1. Clinical Immunology Service, Institute of Medical Pathology, University of Florence, Florence
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  • M. RICCI

    Corresponding author
    1. Clinical Immunology Service, Institute of Medical Pathology, University of Florence, Florence
      Professor M. Ricci. Cattedra di Immunologia Clinica, Istituto di Patologia Medica (Policlinico di Careggi), 50134 Firenze (Italy).
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Professor M. Ricci. Cattedra di Immunologia Clinica, Istituto di Patologia Medica (Policlinico di Careggi), 50134 Firenze (Italy).

Summary

The antigenic composition of D. pteronyssinus extract was investigated by using antisera raised in rabbits by injection of the mile extract in Freund's adjuvant. Immunodiffusion analysis revealed the presence in the mite extract of many antigenic constituents, most of which were also common to human dander and house dust extracts. Four antigenic components not shared by human dander appeared to be common to D.farinae extract.

D. pteronyssinus extract was then fractionated on Sephadex G-75 and DEAE-Sephadex. Immunodiffusion analysis of the most active fraction revealed, after heat-treatment, the presence of three precipitin lines, one of which appeared not to be shared by nutrient medium or by D. farinae extract constituents.

When the anti-mite rabbit serum was fixed to an immunosorbent and incubated with a house dust extract it was constantly able to induce a strong decrease or a complete loss of the allergenicity of the dust extract in man. These results suggest that mite allergen(s) are immunogenic in the rabbit and provide more direct evidence for identity of the allergens in house dust and mite.

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