Allergenic potency of bee antigens measured by RAST inhibition

Authors

  • C. E. ARBESMAN,

    Corresponding author
    1. Allergy Research Laboratory of the Buffalo General Hospital and Department of Medicine and Microbiology of the State University of New York at Buffalo
      Dr Carl E. Arbesman, Allergy Research Laboratory, Buffalo General Hospital, 100 High Street, Buffalo, New York 14203, U.S.A.
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  • R. E. REISMAN,

    1. Allergy Research Laboratory of the Buffalo General Hospital and Department of Medicine and Microbiology of the State University of New York at Buffalo
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  • J. I. WYPYCH

    1. Allergy Research Laboratory of the Buffalo General Hospital and Department of Medicine and Microbiology of the State University of New York at Buffalo
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Dr Carl E. Arbesman, Allergy Research Laboratory, Buffalo General Hospital, 100 High Street, Buffalo, New York 14203, U.S.A.

Summary

The potency of various bee antigens including bee venom, several whole bee body extracts and fractions of bee venom was studied using the RAST inhibition method. As compared to whole bee body extract, bee venom was a much more potent inhibitor of both bee venom and whole body RAST, suggesting that venom has a greater capacity to bind specific bee IgE antibodies. Whole body extracts also varied substantially in their inhibiting activity. Phospholipase A and hyaluronidase were the most potent of the bee venom fractions suggesting their potential use as an assay for standardization of insect extracts.

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