Analysis of the non-immunological activity of allergen extracts in cutaneous tests
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 9, Issue 3, pages 283–291, May 1979
How to Cite
GUERIN, B. and TIOULONG, S. (1979), Analysis of the non-immunological activity of allergen extracts in cutaneous tests. Clinical & Experimental Allergy, 9: 283–291. doi: 10.1111/j.1365-2222.1979.tb01555.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- (Received 24 July 1978; revision received 28 September 1978 and 31 October 1978; accepted for publication 13 November 1978)
The technique proposed by the National Institute for Biological Standards and Control, London (NIBSC, 1977) for the control of allergenic extracts for non-immunological activity in skin tests has proved to be both sensitive and useful. We have observed ‘false positive’ results to substances such as merthiolate or glycerine and also to lyophilised preparations with sugar, notably glucose, as the carrier substance.
Other factors studied in ‘false positive’ reactions to a given extract were pH, osmotic pressure and the presence of traces of endotoxins or histamine. Individually none of these provided satisfactory explanations for the false positive reactions and the use of antagonists or inhibitors of the release of chemical mediators only confirmed the complexity of the non-immunological reaction and the participation of factors other than histamine.