Antibodies to Aspergillus fumigatus in farmers' lung patients measured by enzyme-linked immunosorbent assay (ELISA)

Authors

  • R. A. MÄNTYJÄRVI,

    Corresponding author
    1. Department of Clinical Microbiology, University of Kuopio, Departments of Pulmonary Diseases and Microbiology, University Central Hospital, Kuopio, Finland
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  • P. JOUSILAHTI,

    1. Department of Clinical Microbiology, University of Kuopio, Departments of Pulmonary Diseases and Microbiology, University Central Hospital, Kuopio, Finland
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  • M.-L. KATILA

    1. Department of Clinical Microbiology, University of Kuopio, Departments of Pulmonary Diseases and Microbiology, University Central Hospital, Kuopio, Finland
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Dr R. A. Mäntyjärvi, Department of Clinical Microbiology, University of Kuopio, P.O. Box 138, 70101 KUOPIO 10, Finland.

Summary

Enzyme-linked immunosorbent assay (ELISA) was used to measure antibodies against Aspergillus fumigatus in farmers' lung patients, various other pulmonary diseases and healthy controls. Both the prevalence and the mean titre of antibodies were significantly higher in farmers' lung patients than in the other groups. There was no difference between controls and patients with bronchial asthma or non-allergic pulmonary diseases. On the other hand, in the group of patients with respiratory diseases of undefined aetiology the mean antibody titre was significantly higher than that of the controls. Comparison of ELISA and the precipitin test showed the higher sensitivity of ELISA, but otherwise the two tests were in a close agreement.

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