Flow-cytometric analysis of human basophil degranulation

II. Degranulation induced by anti-IgE, anti-IgG4 and the calcium ionophore A23187

Authors

  • T. NAKAGAWA,

    1. Institute of Clinical Immunology, and Institute of Clinical and Experimental Tumour Research, University of Bern, Bern, Switzerland
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  • B. M. STADLER,

    1. Institute of Clinical Immunology, and Institute of Clinical and Experimental Tumour Research, University of Bern, Bern, Switzerland
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  • D. C. HEINER,

    1. Institute of Clinical Immunology, and Institute of Clinical and Experimental Tumour Research, University of Bern, Bern, Switzerland
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  • F. SKVARIL,

    1. Institute of Clinical Immunology, and Institute of Clinical and Experimental Tumour Research, University of Bern, Bern, Switzerland
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  • A. L. DE WECK

    Corresponding author
    1. Institute of Clinical Immunology, and Institute of Clinical and Experimental Tumour Research, University of Bern, Bern, Switzerland
      Dr A. L. de Weck, Institute of Clinical Immunology, Inselspital. CH-3010, Bern, Switzerland.
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Dr A. L. de Weck, Institute of Clinical Immunology, Inselspital. CH-3010, Bern, Switzerland.

Summary

Quantification of human basophil degranulation induced by anti-IgE, anti-IgG4, and by ionophore was performed using a flow-cytometric system. It was shown that these antibodies and ionophore can degranulate basophils in a dose-dependent manner, and that there is a wide variation in the response of basophils obtained from different individuals to these stimuli. A significant correlation was observed between the degree of degranulation induced by anti-IgE and anti-IgG4, while this was not the case for anti-IgE and ionophore. It was also shown that IgG4 myeloma protein can passively sensitize basophils. In general, degranulating efficacy was in the order of ionophore > anti-IgE >anti-IgG4, both in allergic and non-allergic individuals.

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