An automated particle-counting immunoassay (PACaIA) for serum IgE
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 11, Issue 5, pages 453–461, September 1981
How to Cite
MAGNUSSON, C. G. M., OLLET-CASSART, D. C., MERRETT, T. G. and MASSON, P. L. (1981), An automated particle-counting immunoassay (PACaIA) for serum IgE. Clinical & Experimental Allergy, 11: 453–461. doi: 10.1111/j.1365-2222.1981.tb01619.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- (Received 11 February 1981; accepted for publication 24 March 1981)
Total IgE was determined in 107 sera using a novel, automated, non-isotopic immunoassay called PACIA (particle-counting immunoassay) based on agglutination of anti-IgE coated latex particles by IgE. The IgE values ranged from 10 to 50 000 iu/ml and were compared with results obtained by a conventional radioimmunoassay (RIA) which used a fast double antibody separation technique the coefficient of correlation was 0.985 and the regression line r = 0-82 x+ 130.00.
PACIA had several advantages over the RIA technique: using a sampling rate of 50.hr. results were obtained in 35 min compared to 16-20 hr. no labelled IgE was required and the separation step, which relied on measuring the number of non-agglutinated particles by an optical cell counter, was fully automated. The threshold of sensitivity was 10 iu/ml and the maximal coefficient of variation for between assay precision was 12-9%.