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Ligand binding properties of the basophilic IgE receptor of normal and allergic individuals
Article first published online: 27 APR 2006
DOI: 10.1111/j.1365-2222.1982.tb01630.x
1365-2222/asset/cover.gif?v=1&s=363d6ac7042b7535760df61b7393fd8e0a08e85f "Clinical & Experimental Allergy")
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How to Cite
WEICHMAN, B. M. and MUCCITELLI, R. M. (1982), Ligand binding properties of the basophilic IgE receptor of normal and allergic individuals. Clinical & Experimental Allergy, 12: 121–129. doi: 10.1111/j.1365-2222.1982.tb01630.x
Publication History
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- (Received 24 July 1981; accepted 9 September 1981)
- Abstract
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- Cited By
Summary
The interaction of [125I]-human myeloma IgE with the human basophilic IgE receptor of normal and allergic individuals was quantitated by direct ligand binding analysis after removal of endogenous IgE. Using a mixed leucocyte preparation containing 2 to 5% basophils, the [125I]-IgE binding was specific for human IgE, paralleled the presence of basophils in the cell preparation, and was described by a time course similar to that reported for passive sensitization experiments. The Kd values were 4.0 to 13.2 nM for the normal individuals with 1.5 to 8.4 × 105 receptors per basophil, while the IgE receptor of allergic individuals was described by Kd values of 4.2 to 11.7 nM and 3.0 to 4.7 × 105 sites per basophil. Based upon the eleven individuals studied, these results suggest that the binding properties of the IgE receptor of normal and allergic individuals are similar.