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Clinical & Experimental Allergy

Observations on high spontaneous release of histamine from leucocytes in vitro

Authors

  • C. D. MAY,

    Corresponding author
    1. Departments of Pediatrics, National Jewish Hospital and Research Center, and University of Colorado Medical School, Denver, Colorado
      Dr Charles D. May, National Jewish Hospital, Department of Pediatrics, 3800 East Colfax Avenue, Denver, Colorado 80206, USA.
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  • LINDA REMIGIO

    1. Departments of Pediatrics, National Jewish Hospital and Research Center, and University of Colorado Medical School, Denver, Colorado
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Dr Charles D. May, National Jewish Hospital, Department of Pediatrics, 3800 East Colfax Avenue, Denver, Colorado 80206, USA.

Summary

Clinical and laboratory observations are provided on high, spontaneous, in vitro histamine release (HSHR) from leucocytes (basophils), meaning without addition of antigen or other inducers of histamine release. HSHR has been observed predominantly in persons with a high degree of reaginic sensitivity to food; the leucocytes of forty-eight of fifty-eight individuals (83%), who had positive reactions to foods in double-blind challenges, exhibited HSHR. HSHR resembles antigenic release in a wide variety of characteristics. HSHR does not occur in vivo but only in vitro after compaction of leucocytes by centrifugation at room temperature. An indispensible requirement for HSHR to occur appears to be a high degree of reaginic sensitization.

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