Histamine content of sputum from patients with asthma and chronic bronchitis
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 12, Issue 1, pages 19–27, January 1982
How to Cite
BRYANT, D. H. and PUI, A. (1982), Histamine content of sputum from patients with asthma and chronic bronchitis. Clinical & Experimental Allergy, 12: 19–27. doi: 10.1111/j.1365-2222.1982.tb03122.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- (Received 12 May 1981; accepted for publication 5 August 1981)
Studies using the guinea pig ileum bioassay technique have previously shown that patients with either asthma or chronic bronchitis have increased amounts of histamine in their sputum. Because of the limitations of this assay technique, a spectrofluorometric method for measuring the histamine content of sputum was developed. Using this technique it was found that the mean histamine concentration of sputum from patients with bronchogenic carcinoma or pneumonia was not significantly different from the mean value for sputum collected during a remission phase from patients with either chronic bronchitis or asthma. However, the mean values in patients with asthma or chronic bronchitis during an exacerbation were significantly higher and returned to basal values as the airways obstruction improved. There was no significant difference in sputum histamine concentration between those patients with extrinsic atopic asthma and those with late onset cryptogenic asthma during an exacerbation although the value in patients with an exacerbation of chronic bronchitis was significantly less.
These results indicate that mediators of immediate hypersensitivity may be implicated in the pathogenesis of airways obstruction in disorders other than extrinsic atopic asthma and suggest that histamine release may occur in non-atopic subjects in response to non-antigenic stimuli.