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In vitro IgE-secreting cells in man I. Mitogen-independent and -dependent subpopulations
I. Mitogen-independent and -dependent subpopulations
Article first published online: 27 APR 2006
DOI: 10.1111/j.1365-2222.1985.tb02256.x
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How to Cite
MATSUMOTO, T., YAMAGUCHI, K., SHIMAMURA, M. and HARADA, Y. (1985), In vitro IgE-secreting cells in man I. Mitogen-independent and -dependent subpopulations. Clinical & Experimental Allergy, 15: 61–66. doi: 10.1111/j.1365-2222.1985.tb02256.x
Publication History
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- (Received 18 June 1982: accepted far publication 16 July 1982)†
- Abstract
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Summary
In vitro IgE secretion by atopic and normal peripheral-blood lymphocytes was examined in culture with pokeweed mitogen or Staphylococcus aureus strain Cowan-I (StaCw) or without mitogen. IgE secreted in culture supernatants was measured with double antibody radioimmunoassay. Enumeration of IgE-secreting cells was made by a protein-A plaque assay. IgE was detected in increasing quantities in supernatants of cultured lymphocytes without mitogen up to the 12th day. IgE-plaque-forming cells were formed by the lymphocytes in large numbers on days 4–7 in cultures with mitogen. These results suggest that not only mitogen-independent but also mitogen-dependent subpopulations may exist in the IgE-secreting cells.