Deparlment of Immunology, Institute of Child Health. 30 Guilford Street. London WCIN lEH. U.K.
The allergens of dog. I. Identification using crossed radio-immunoelectrophoresis
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 19, Issue 2, pages 183–190, March 1989
How to Cite
FORD, A. W., ALTERMAN, L. and KEMENY, D. M. (1989), The allergens of dog. I. Identification using crossed radio-immunoelectrophoresis. Clinical & Experimental Allergy, 19: 183–190. doi: 10.1111/j.1365-2222.1989.tb02362.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 7 September 1987; revised 26 September 1988; accepted 3 October 1988.
The antigens present in an extract of dog hair and dander were examined by crossed immunoelectrophoresis (CIE) and the IgE-binding allergens by crossed radio-immunoelectrophoresis (CRIE), respectively, using sera from 60 British and Finnish animal-allergic subjects. The extract was comprised of a minimum of 28 antigens, 11 of which were common to dog serum. IgE antibody in the sera of the dog-sensitive patients bound to 21 of the 28 antigens at varying frequencies and intensities. Binding of any intensity occurred most frequently to two serum proteins: antigen 23 (IgG) binding IgE in 88% of cases, and antigen 3 (dog serum albumin, DSA) in 77% of cases. Dander antigen 8 bound in 63% and antigen 1 in 42% of the sera. Strong IgE binding, however, was most commonly associated with dander antigen 8 followed by antigens 1 and 23 (IgG) then 3 (DSA). The ranking of the antigens as allergens was similar for the two populations except that DSA was more important for the British than for the Finnish subjects.