Natural anti-IgE auto-antibodies interfere with diagnostic IgE determination
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 20, Issue 3, pages 295–309, May 1990
How to Cite
VASSELLA, C. C., DE WECK, A. L. and STADLER, B. M. (1990), Natural anti-IgE auto-antibodies interfere with diagnostic IgE determination. Clinical & Experimental Allergy, 20: 295–309. doi: 10.1111/j.1365-2222.1990.tb02687.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 21 June 1989; revised 1 October 1989; accepted 13 December 1989.
A simple method is described which enables measurement of anti-IgE antibodies in free form as well as in immune complexes of IgE and anti-IgE. Anti-IgE antibodies were purified from serum of one selected blood donor with highly elevated levels of such auto-antibodies. These purified anti-IgE auto-antibodies inhibited the measurement of myeloma IgE. Purification also revealed that 98% of the subject's serum IgE was masked by anti-IgE auto-antibodies. Our data suggest that IgE determinations in sera containing anti-IgE antibodies might be underestimated.