cDNA encoding the major mite allergen Der f II

Authors


Dr W. R. Thomas, Western Australian Research Institute for Child Health, Princess Margaret Hospital, GPO Box D184. Perth. Western Australia 6001.

Summary

cDNA encoding the major house dust mite allergen Der f II from Dermatophagoides farinae was amplified using the polymerasc chain reaction and cloned into E. coli. It encoded a 129-residue protein with a calculated molecular weight of 14021 D and had the expected high homology (88%) with Der p II including the absence of N-glycosylation sites and conserved cysteine residues. These results are consistent with the high degree of antibody crossreactivity and may help identify the differences in T-cell epitopes revealed for these molecules so far.

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