Clinical & Experimental Allergy

Monoclonal antibodies bind identically to both spores and hyphae of Aspergillus fumigatus

Authors

  • K. E. REIJULA,

    Corresponding author
    1. Research Service, VA Medical Center, and the Department of Medicine, Allergy-Immunology Division, Medical College of Wisconsin, Milwaukee, WI, U.S.A.
      and present address: Dr K. Reijula, Oulu Regional Institute of Occupational Health, PO Box 451, SF-90101 Oulu, Finland.
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  • V. P. KURUP,

    1. Research Service, VA Medical Center, and the Department of Medicine, Allergy-Immunology Division, Medical College of Wisconsin, Milwaukee, WI, U.S.A.
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  • A. KUMAR,

    1. Research Service, VA Medical Center, and the Department of Medicine, Allergy-Immunology Division, Medical College of Wisconsin, Milwaukee, WI, U.S.A.
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  • J. N. FINK

    1. Research Service, VA Medical Center, and the Department of Medicine, Allergy-Immunology Division, Medical College of Wisconsin, Milwaukee, WI, U.S.A.
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and present address: Dr K. Reijula, Oulu Regional Institute of Occupational Health, PO Box 451, SF-90101 Oulu, Finland.

Summary

Immunoelectron microscopy (IEM) was used to determine the binding of six monoclonal antibodies (MoAbs) produced against Aspergillus fumigatus antigens present on or within the conidia and hyphae of the fungus. Antigen-antibody complexes were demonstrated in EM using labelled colloidal gold particles (15 nm). Three out of 6 MoAbs (C9, F12 and H10) reacted only with the cytoplasmic components of A. fumigatus while the remaining three (B12, F6G5 and D6E6) showed reactivity to both cytoplasm and cell wall of the conidia and hyphae. The results indicate that IEM is of considerable value in determining and selecting monoclonal antibodies having specific reactivity with diverse antigenic components.

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