Heterogeneity of IgE antibody response to reactive dye in sera from four different sensitized workers
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 22, Issue 6, pages 606–610, June 1992
How to Cite
HONG, C.-S. and PARK, H. S. (1992), Heterogeneity of IgE antibody response to reactive dye in sera from four different sensitized workers. Clinical & Experimental Allergy, 22: 606–610. doi: 10.1111/j.1365-2222.1992.tb00176.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 4 September 1991; revised 21 November 1991; accepted 2 December 1991.
We studied RAST and RAST inhibition tests to black GR, the most frequent sensitizer among several reactive dyes in our previous study, in order to evaluate the specificity of IgE antibodies to hapten or new antigenic determinants and the crossreactivity between two reactive dyes, black GR and orange 3R, in sera from four different sensitized workers. RAST inhibition studies with black GR-human serum albumin (HSA) conjugate discs demonstrated that black GR-HSA conjugates were the most effective inhibitors. Orange 3R-HSA conjugates, unconjugated forms of black GR and orange 3R were weak inhibitors in two patients and non-inhibitory in one patient, whereas they caused strong dose-dependent inhibitions in one patient. These results suggested that the IgE response to black GR-HSA conjugates might be heterogenous and the cross-reactivity between two reactive dyes differed from one patient to another.