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Summary

Stability of Candida albicans allergens was studied under various storage conditions Lyophilized extract was reconstituted with human serum albumin glycerol-free and in the presence of 10% or 50% glycerol and stored at various temperatures for different time periods. All extracts were tested at the same time with immunoblotting using C albicans allergic patient sera and galactosidase-labelled anti-IgE. The highest number of detected allergens in the immunoblotting pattern was found in the presence of 50% glycerol at +6 C. The most important allergen of $$ the 46 kD protein allergen was stable up to 10 weeks at +6 $$ C in the presence of 50% glycerol but thereafter began to lose its IgE-binding capacity. After 30 weeks more than 50% of the IgE binding had disappeared. The 27 kD protein, another important allergen, was also labile but retained the allerg$$ better than the 46kD one. The 29kD protein allergen was stable at all storage conditions, except +3$$C tested even after one year. More than 6 months storage at +6C or higher temperature is however unacceptable even in the presence of the 50% glycerol. These findings have particular importance in the diagnosis and treatment of allergic diseases