Molecular cloning of a house dust mite allergen with common antibody binding specificities with multiple components in mite extracts

Authors

  • H-D. SHEN,

    1. The Western Australian Research Institute for Child Health, Perth, Western Australia 600 J, Australia and Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, Republic of China
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  • K-Y. CHUA,

    1. The Western Australian Research Institute for Child Health, Perth, Western Australia 600 J, Australia and Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, Republic of China
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  • K-L. LIN,

    1. Department of Paediatrics, National Taiwan University, College of Medicine, Taipei. Republic of China
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  • K-H. HSIEH,

    1. Department of Paediatrics, National Taiwan University, College of Medicine, Taipei. Republic of China
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  • W. R. THOMAS

    Corresponding author
    1. The Western Australian Research Institute for Child Health, Perth, Western Australia 600 J, Australia and Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, Republic of China
      Dr W. R. Thomas, The Western Australian Research Institute for Child Health, GPO Box D184, Perth, Western Australia 6001, Australia.
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Dr W. R. Thomas, The Western Australian Research Institute for Child Health, GPO Box D184, Perth, Western Australia 6001, Australia.

Summary

Plaque radio-immuno assay has been used to isolate an FgE-binding clone from a lambda gt11 library of Dermatophagoides pteronyssinus cDNA, The clone HD6 contained DNA encoding a 215 residue protein which contained a predicted 17 amino acid residue leader sequence, no cysteines and a single W-glycosylation site. The 198 residue mature protein would have a predicted MW of 22 177 D. No homologues were found in searches of the data banks. Sera from 14/38 allergic children reacted strongly with the polypeptide produced by the clone (37%). Skin tests showed reactivity in 16/30 (53%) allergic patients and 0/10 of controls. Affinity purification of rabbit antibodies with the clone showed that antibodies to the polypeptide had specificities to multiple products in mite extracts corresponding to components of Mr 29, 27 and 24 K by Western blotting. Absorption studies of IgE in allergic serum indicated further entities at 13 and 11.5 kD, It is proposed to name this allergen Der p VII.

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