Standardization of pollen allergens of Parthenium hysterophoms and selection of an in-house reference extract

Authors

  • P. SRIRAMARAO,

    1. Lahoratory of Immunology and Allergic Diseases, Department of Biochemistry
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    • La Jolla lnslitule of Experimental Medicine, 11099N. Torrey Pines Road, La Jolla, CA 92037, U.S.A.

  • H. SCHRODER,

    1. Lahoratory of Immunology and Allergic Diseases, Department of Biochemistry
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    • Visiting scientist from Pharmacia Diagnostics AB, Uppsala, Sweden.

  • B. S. SUBBA RAO,

    Corresponding author
    1. Lahoratory of Immunology and Allergic Diseases, Department of Biochemistry
    2. Health Centre. Indian Institute of Science
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  • O. PRAKASH,

    1. Lahoratory of Immunology and Allergic Diseases, Department of Biochemistry
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    • Division of Medicine, St Martha's Hospital, Bangalore, India

  • P. V. SUBBA RAOH

    1. Lahoratory of Immunology and Allergic Diseases, Department of Biochemistry
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    • Visiting scientist from Pharmacia Diagnostics AB, Uppsala, Sweden.


  • Prof. P.V, Subba Rao. Laboratory of Immunology and Allergic Diseases. Department of Biochemistry. Indian Institute of Science. Bangalore 560012, India.

Prof. P.V. Subbu Rao, Laboratory of Immunology and Allergic Diseases, Department of Biochemistry, Indian Institute of Science. Bangalore 560012, India.

Summary

A standardized in-house reference extract from the pollen of Parthenium hysterophoms, which is responsible for the high incidence of allergic rhinitis in India, was generated and examined by skin test, radio-allergosorbent test inhibition and isoelectric focusing. Parthenium reference allergen discs and positive reference serum were also generated. These reference reagents could not only be used for the quantitation of Panhenium-specific IgE in the sera of rhinitis patients but also for the evaluation of allergenic activity (relative potency and lot-to-lot variation) of different batches of Parthenium pollen.

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