Seasonal enhancement of IL-4 induced IgE synthesis by peripheral blood mononuclear cells of atopic patients

Authors

  • R. GAGNON,

    Corresponding author
    1. Centre de Recherche en Inflammation. Immunologie et Rhumatologie. Centre de Recherche du Centre Hospitalier de I'Université Laval. Ste-Foy. (Quebec) Canada
      Dr R. Gagnon. Centre de Recherche en Inflammation. Immunologie LM Rhumulologic. Room 9800, Centre de Recherche du Cenlre Hospilalier dc rUniversile Laval, 2705 boul. Laurier, Ste-Foy, (Quebec) Canada GIV 4G2.
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  • JING LIAN,

    1. Centre de Recherche en Inflammation. Immunologie et Rhumatologie. Centre de Recherche du Centre Hospitalier de I'Université Laval. Ste-Foy. (Quebec) Canada
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  • Y. BOUTIN,

    1. Centre de Recherche en Inflammation. Immunologie et Rhumatologie. Centre de Recherche du Centre Hospitalier de I'Université Laval. Ste-Foy. (Quebec) Canada
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  • J. HÉBERT

    1. Centre de Recherche en Inflammation. Immunologie et Rhumatologie. Centre de Recherche du Centre Hospitalier de I'Université Laval. Ste-Foy. (Quebec) Canada
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Dr R. Gagnon. Centre de Recherche en Inflammation. Immunologie LM Rhumulologic. Room 9800, Centre de Recherche du Cenlre Hospilalier dc rUniversile Laval, 2705 boul. Laurier, Ste-Foy, (Quebec) Canada GIV 4G2.

Summary

The role of IL-4 on IgE synthesis has been well established. IL-4 has been shown to promote IgE production by B cells from atopic and non-atopic donors. In this study, the effects of natural exposure to pollens on IL-4-indueed IgE synthesis by peripheral blood mononuclear cells of atopic and non-atopic donors were examined. The results confirm production of IgE in an IL-4 dose-dependent manner by PBMC cultures of these two groups. When cultures were performed out of the pollen season, following stimulation by IL-4, no significant difference was observed between the levels of IgE produced by PBMC of atopic and non-atopic donors. In contrast, upon natural exposure to pollens, significant higher levels of IgE were measured in the atopic group than in the non-atopic one. These results show that the policn season infiuences the IL-4-induced IgE synthesis by PBMC of allergic patients and are in keeping with seasonal rise of specific IgE antibodies.

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