Endotoxin and pro-inflammatory cytokines stimulate endothelin-I expression and release by airway epithelial cells
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 24, Issue 4, pages 330–336, April 1994
How to Cite
NAKANO, J., TAKIZAWA, H., OHTOSHI, T., SHOJI, S., YAMAGUCHI, M., ISHII, A., YANAGISAWA, M. and ITO, K. (1994), Endotoxin and pro-inflammatory cytokines stimulate endothelin-I expression and release by airway epithelial cells. Clinical & Experimental Allergy, 24: 330–336. doi: 10.1111/j.1365-2222.1994.tb00243.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 12 May 1993; revised 2 August 1993; accepted 4 November 1993.
Endothelin is a potent bronchoconstrictor peptide first identified as a novel vasoconstrictor produced by vascular endothelial cells. Recent reports suggest that airway epithelial cells are also capable of releasing this active peptide. To investigate the regulatory mechanism of endothelin expression, we studied the effects of endotoxin and pro-inflammatory cytokines such as interleukin-1 and tumour necrosis factor on the expression and release of endothelin-1 by airway epithelial cells. Both endotoxin and the cytokines stimulated endothelin-1 release by human bronchial epithelial cells. Northern blot analysis showed increased expression of preproendothelin-1 mRNA by these factors. These results suggested that airway epithelial cells might play a role in the local airway smooth muscle tone through the production of endothelin, which might be upregulaled by inflammatory products in the airways.