Clinical & Experimental Allergy

Isolation and denomination of an important allergen in baking additives: α-amylase from Aspergillus oryzae (Asp o II)

Authors

  • X. BAUR,

    Corresponding author
    1. Professional Associations' Research Institute for Occupational Medicine (BGFA) at the Ruhr University of Bochum, Bochum, Germany
      Dr X. Baur, BGFA, Gilsingstr. 14, D-44789 Bochum, Germany
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  • Z. CHEN,

    1. Professional Associations' Research Institute for Occupational Medicine (BGFA) at the Ruhr University of Bochum, Bochum, Germany
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  • I. SANDER

    1. Professional Associations' Research Institute for Occupational Medicine (BGFA) at the Ruhr University of Bochum, Bochum, Germany
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Dr X. Baur, BGFA, Gilsingstr. 14, D-44789 Bochum, Germany

Abstract

Abstract. The commercially available α-amylase from Aspergillus oryzae which is widely used as a baking additive was compared with a highly purified enzyme preparation. We used enzyme allergosorbent test (EAST), EAST inhibition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focussing, immunoblotting, and N-terminal amino acid sequencing to characterize the causative allergen. Our screening comprised 89 partially selected bakers. Forty-three (48%) of them had work-related respiratory symptoms; 14 (32%) of whom were sensitized to the baking additive. Significant immunological differences could not be found between crude and purified sample with the exception that the latter one produced nearly twice as high antibody values. Iodine starch staining demonstrated that the component which was exclusively or predominantly bound by IgE antibodies of symptomatic bakers represents the active α-amylase. According to the International Union of Immunological Societies (IUIS) nomenclature, the term Asp o II is suggested for this important occupational allergen.

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