Dissociation of airway responsiveness and bronchoalveolar lavage (BAL) cell composition in sensitized guinea–pigs after daily inhalation of ovalbumin
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 24, Issue 7, pages 682–689, July 1994
How to Cite
HEUER, H. O., WENZ, B., JENNEWEIN, H. M. and URICH, K. (1994), Dissociation of airway responsiveness and bronchoalveolar lavage (BAL) cell composition in sensitized guinea–pigs after daily inhalation of ovalbumin. Clinical & Experimental Allergy, 24: 682–689. doi: 10.1111/j.1365-2222.1994.tb00973.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 1 November 1993; revised 8 February 1994; accepted 12 March 1994.
Summary. The association between inflammatory cell influx, cell activation status and change of airway responsiveness to acelylcholine (ACh) after daily inhalation of ovalbumin (OA) in sensitized guinea–pigs was investigated. Starting 3 weeks after sensitization (OA at 50mg/kg s.c. + i.p.) guinea–pigs were exposed daily to 2% OA (10min: undercover of 0.5Smg/kg mepyramine i.p. 15min before OA) for 2 weeks. Concentration–response curves (CRCs) for inhaled ACh were performed 24 h after the last OA–challenge and 24 h after another single OA–inhalation 1 week later. CRCs for inhaled ACh were neither affected 24 h after the last OA challenge (daily for two weeks) nor 24 h after another OA–inhalation one week later. In contrast, bronchoalveolar lavage (BAL) from repeatedly OA– sensitized/–challenged guinea–pigs immediately after the last CRC showed a significant increase of total cell count by about tenfold and increases in eosinophils by about 20–fold, neutrophils by 30–fold, macrophages by about fivefold and lymphocytes by about tenfold (P < 0.05. multiple Wilcoxon–test). In contrast, markers of cell activation (EPO, MPO) were significantly decreased (P < 0.05). Methylprednisolone almost completely prevented these changes in increased cell numbers and decreased cell activation (vs OA contr., P < 0.05). The lack of increased airway hyperresponsiveness despite a massive inflammatory cell influx suggests other factors controlling airway responsiveness than inflammation.