Sensitivity to two major allergens (Cry j I and Cry j II) in patients with Japanese cedar (Cryptomeria japonica) pollinosis
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 25, Issue 9, pages 848–852, September 1995
How to Cite
HASHIMOTO, M., NIGI, H., SAKAGUCHI, M., INOUYE, S., IMAOKA, K., MIYAZAWA, H., TANIGUCHI, Y., KURIMOTO, M., YASUEDA, H. and OGAWA, T. (1995), Sensitivity to two major allergens (Cry j I and Cry j II) in patients with Japanese cedar (Cryptomeria japonica) pollinosis. Clinical & Experimental Allergy, 25: 848–852. doi: 10.1111/j.1365-2222.1995.tb00027.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 20 October 1994; revised 25 January 1995; accepted 21 March 1995.
- histamine release;
Background: Japanese cedar (Cryptmeria japonica: CJ) pollinosis is one of the most important allergic diseases in Japan. Recently, the second major allergen (Cry j II) was isolated from CJ pollen. There have been no prevalence studies of sensitivity to Cry j I and Cry j II among a large number of patients with pollinosis.
Objective: This study was conducted to evaluate the prevalence of sensitivity to Cry j I and Cry j II. We measured specific IgE antibodies to these allergens in the sera of 145 patients. Furthermore, comparison of the sensitivity to Cry j I and Cry j II was examined by the hisiamine release assay.
Methods: Specific IgE antibodies to Cry j I and Cry j II were assayed by a fluorometric ELISA. Allergen-specific histamine release was measured by a radioimmunoassay kit,
Results: More than 90% of 145 patients had specific IgE antibodies to both allergens. the remainder had specific IgE to either one or the other. There were seasonal changes in the level of specific IgE. The changes in the levels of anti-Cry j II IgE antibodies were parallel to those of anti-Cry j I IgE. The histamine release assay with leucocytes from the patients demonstrated that the allergenic potency of the two allergens is almost the same.
Conclusion: Cry j II is an as important a major allergen as Cry j I.