Clinical & Experimental Allergy

Characterization of allergenic components from house dust mite Dermatophagoides siboney. Purification of Der s 1 and Der s 2 allergens

Authors


Dr R. Ferrándiz. Department of Paediatrics. Level 14. Linköping University Hospital. S-581 85 Linköping. Sweden.

Abstract

Background Allergic reactions to house dust miles of the genus Dermatophagoides play an important role in the pathogenesis of asthma and other atopic diseases. Dermatophagoides siboney has been described as a species from Cuba. Together with D. pteronyssinus and Blomia tropicalis, it is frequently found in house dust from homes of asthmatics.

Objective The aim of this study was to investigate the allergenic composition from the house dust mite D. siboney.

Methods The charactcrization of D. siboney extract was performed by SDS-gPAGE and immunoblotting. Purification of individual components was performed by affinity ehromatography.

Results At least 16 components between 13 and 98 k Da stained by Coomassie Blue were found. Using a panel of 35 sera from a topic mile sensitive patients 13 components reacted to different extent with patient IgE. Two components, 25 and 14 kDa, bound to specific IgE strongly and frequently, i.e. 80 and 91% of the patients, respectively. Affinity ehromatography using crossreacting monoclonal antibodies to group 1 and 2 allergens resulted in purified preparations of 25 and 14 kDa proteins, which showed IgE-binding with the majority of the human sera when tested by immune-dot.

Conclusion Based on the IgE binding profile of D. siboney and on the capacity to react with crossreacting monoclonal antibodies for groups I and 2, it is proposed to name these two allergens, 25 and 14 k Da, Der s 1 and Der s 2, respectively.

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