Blood inflammatory response to inhaled endotoxin in normal subjects
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 25, Issue 1, pages 73–79, January 1995
How to Cite
MICHEL, O., DUCHATEAU, J., PLAT, G., CANTINIEAUX, B., HOTIMSKY, A., GERAIN, J. and SERGYSELS, R. (1995), Blood inflammatory response to inhaled endotoxin in normal subjects. Clinical & Experimental Allergy, 25: 73–79. doi: 10.1111/j.1365-2222.1995.tb01005.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 19 October 1993; revised 4 January 1994; accepted 25 July 1994.
Previously we have reported that in asthmatics an inhalation of 20 μg lipopolysaccharide (LPS) produces a bronchial obstruction associated with an inflammatory blood response. The aim of the present study was to evaluate this response in normal subjects. Eight normal non-atopic subjects were challenged by inhalation of a solution containing 20 μg LPS (from Escherichia coli 026:B6) a week after bronchial challenge with control solution. The lung function response was evaluated by the changes in forced expiratory volume in one second (FEV1), in specific conductance and in airway resistance while the blood inflammatory response was evaluated by serial measures of total white blood cells (WBC) and polymorphonuclear neutrophils (PMN) count, luminol enhanced-chemiluminescence (luminol-CL, as a marker of the PMN degree of activation), C-reactive protein (CRP), haptoglobin, complement fraction C3, tumour necrosis factor-α (TNF-α) and adrenocorticotropic hormone (ACTH). No response in lung function was observed for 6 h after the LPS inhalation. The count in WBC and PMN increased 300 (P < 0.01) and 360 (P < 0.01) min after the LPS challenge associated with an increase in the level of luminol-CL (P < 0.001). This rise in luminol-CL level was significant at 120 min (P < 0.05) before any change in the PMN count. After 24 and 48 h the acute-phase protein CRP raised significantly (P < 0.01), the other proteins C3 and haptoglobin being unchanged. A slight increase in ACTH was observed 240 and 360 min (P < 0.05) after the LPS challenge while the TNFα detectable level was not modified. In conclusion, in normal subjects, inhalation of a pro-inflammatory agent is able to induce a systemic inflammatory response in the absence of any effect on lung mechanics, while in asthmatics the same bronchial challenge has been reported to induce a similar blood inflammation associated with a significant response in lung function.