Podocarpus gracilior and Callitris verrucosa—newly identified allergens that crossreact with Cupressus sempervirens
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 25, Issue 5, pages 456–460, May 1995
How to Cite
BAR DAYAN, Y., KEYNAN, N., WAISEL, Y., PICK, A. I. and TAMIR, R. (1995), Podocarpus gracilior and Callitris verrucosa—newly identified allergens that crossreact with Cupressus sempervirens. Clinical & Experimental Allergy, 25: 456–460. doi: 10.1111/j.1365-2222.1995.tb01077.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 30 December 1992; revised 24 June 1994; accepted 1 December 1994.
Thirty-six symptomatic patients, with positive skin reactions to Cupressus sempervirens pollen extract were skin-tested with pollen extracts of Podocarpus gracilior and Callitris verrucosa, of these 17 (47%) had positive responses to P. gracilior, nine (25%) to C. verrucosa, and six (17%) to both. None of the non-atopic healthy controls had positive reactions to either of the extracts. Radioallergosorbent test (RAST)-inhibition studies were performed with pooled sera from three patients. Fifty per cent inhibition was obtained with 11 μg protein of C. sempervirens, 54μg of P. gracilior, and 71 μg of C. verrucosa; however, when pollen extract of Olea europaea, an unrelated allergen, was tested, 265μg protein were needed to obtain 50% inhibition. One-dimension sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of pollen extracts from the three species revealed that they had several very similar protein bands. Using Western blot analysis, several closely related IgE binding proteins were identified in the three species. It was concluded that the pollen grains of P. gracilior and of C. verrucosa are potentially allergenic. The presence of common allergenic proteins indicate partial crossreactivity with C. sempervirens.