Allergy to olive pollen: T-cell response from olive allergic patients is restricted by DR7-DQ2 antigens

Authors


Dr C. Lahoz. Immnology Department. Function Jimenez Diaz, Av. Reyes Catolicos 2. 28040 Madrid. Spain.

Summary

Backgound We have recently described the association between the IgE antibody response to Ole e I (the major antigen from olive tree pollen) and the DR7-DQ2 haplotype in a Spanish population.

Objeectivc and methods Due to the linkage disequilibrium between DR7 and DQ2, and thus the dillicult distinction between the role of these two antigens in the T-cell activation response, we decided to solve this question by two approaches:

1. The study of another ethnic group, individuals of Arabic origin, with a presumably distinct disequilibrium linkage between DR and DQ antigens. Genomic DNA typing was performed in 46 subjects (allergic and non-allergic) by Restriction Fragment Length Polytnotphism (RFLP) and results showed that patients with specific IgE antibodies α-Ole e I, were DR7 and or DQ2. These data show a similar restriction pattern to those previously described for Spanish patients. The phenolypic frequency of DR7 antigen is significantly greater than in the non-allergic population, with a corrected P(PJ value of O.O.3

2 The analysis of the genetic requirements of Ole e I response, using T-cell lines speific for this antigen. This was first carried out by blocking the proliferative response of these T-cell lines with specific anti-human HLA class II antibodies and then testing the genetic restriction of this response using a panel of histoeompatible and histoincompatible Antigen Presenting Cells (APCs). Both experiments corroborate the hypothesis that DR7 and DQ2 are implicated in the recognition o(Ole e I.

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