This study was supported by the National Health and Mrdical Research Council of Australia.
Induction of hyperresponsiveness in human airway tiss by neutrophils — mechanism of action
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 26, Issue 5, pages 549–556, May 1996
How to Cite
ANTICEVICH, S. Z., HUGHES, J. M., BLACK, J. L. and ARMOUR, C. L. (1996), Induction of hyperresponsiveness in human airway tiss by neutrophils — mechanism of action. Clinical & Experimental Allergy, 26: 549–556. doi: 10.1111/j.1365-2222.1996.tb00575.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 5 June 1995; revised 17 October 1995; accepted 27 October 1995.
- human airways;
Background The two main features of asthtna are bronchial hyperresponsiveness and inflammation. The inflammatory response in asthma consists of infiltration and activation of a variety of inflammatory cells including neutrophils. Our previous studies have shown that stimuhited neutrophil supernatants cause hyperresponsiveness of human bronchial tissue in vitro.
Objective To investigate the effect of the sensitization status of the tissue and the albumin concentration used to prepare supernatants on the response of human bronchial tissue to stimulated neutrophil supernatants.
Methods Neutrophil supernatants were prepared from human isolated blood in the presence of varying concentrations of albumin (0%, 0.1% and 4%), Neutrophil supernatants were added to sensitized and non-sensitized human isolated bronchial tissue which was stimulated with electrical field stimulation (EFS) (20 s every 4min). Receptor antagonists specific for the prostaglandin and thromboxane (10−7M GR3219I), platelet activating factor (10−6M WEB 2086), leukotriene D4 (10−6M MK-679) and neurokinin A (10−7M SR48968) receptors were used to identify neutrophil products responsible for the effects observed in the bronchial tissue. Results In non-sensitized human bronchial tissue, stimulated neutrophil supernatants induced a direct contraction in the presence of 0% and 0.1 % but not 4% albumin. This contraction was due to leukotriene D4 as MK-679 completely inhibited the contraction. In contrast, stimulated neutrophil supernatants increased responsiveness of sensitized human bronchial tissue to EFS. The increased responsiveness was observed only in the presence of 0.1% albumin, with the site of modulation likely to be prejunctional on the parasympathetic nerve. The increased responsiveness was not inhibited by any of the antagonists tested.
Conclusion Sensitization status of the tissue and albumin concentration effect the responsiveness of human bronchial tissue to stimulated neutrophil supernatant. Our results suggest a possible role for neutrophils in hyperresponsiveness.