Immune effector cells induced by complete Freund's adjuvant exert an inhibitory effect on antigen-specific type 2 T helper responses

Authors

  • Y.-H. CHUANG,

    1. Graduate Institute of Immunology, National Taiwan University, Taipei, Taiwan, Republic of China
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  • B.-L. CHIANG,

    Corresponding author
    1. Graduate Institute of Immunology, National Taiwan University, Taipei, Taiwan, Republic of China
    2. Graduate Institute of Clinical Medicine, National Taiwan University, Taipei, Taiwan, Republic of China
    3. Department of Pediatrics, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China
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  • C.-C. CHOU,

    1. Department of Pediatrics, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China
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  • K.-H. HSIEH

    1. Department of Pediatrics, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China
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Dr Bor-Luen Chiang, Department of Pediatrics, College of Medicine, National Taiwan University, No. 7 Chung-Shan S. Road. Taipei, Taiwan, Republic of China.

Summary

Background II has hecn well documented that environmental factors such as antigenpresenting cells and related cytokines could affect the development of T helper cells.

Objective The purpose of this study is to investigate the effect of different adjuvants on T cell development.

Methods Ovalbumin (OVA) combined with aluminum hydroxide (Alum) plus pertussis toxin (PT) or complete Freund's adjuvant (CFA) were used to sensitize mice; the production of IgG and IgE anli-OVA antibodies was then followed. In addition, OVA-specific proliferative responses and cytokine production by spleen cells were also investigated.

Results The data showed that the adjuvants themselves could modify the pattern of immune response: (1) IgG2a > anti-OVA antibody was higher in mice sensitized with OVA + CFA compared to that of mice sensitized with OVA + Alum + PT; (2) the ratio of IFN-γ/IL-4 produced by OVA-stimutated spleen cells was higher in mice sensitized with OVA + CFA than that of mice sensitized with OVA + Alum + PT; (3) increased percentage of γδ T cells was noted in the peritoneal exudate cells of OVA + CFA immunized mice; and (4) the immune response of mice sensitized wilh OVA + Alum+ PT was inhibited by the adoptively transferred ascitic cells from OVA + CFA immunized mice.

Conclusion In general, the data suggested higher IgG2a and the ratio of IFN-γ/IL-4 was noted In mice sensitized with OVA + CFA. Further elucidation of the regulatory mechanism of allergen-specific T helper cells development and exploration of possible agents for inmiunotherapy might shed light on the management of atopic diseases.

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