Altered compartimentalization of transforming growth factor-β in asthmatic airways
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 27, Issue 4, pages 389–395, April 1997
How to Cite
MAGNAN, A., RETORNAZ, F., TSICOPOULOS, A., BRISSE, J., VAN PEE, D., GOSSET, P., CHAMLIAN, A., TONNEL, A. B. and VERVLOET, D. (1997), Altered compartimentalization of transforming growth factor-β in asthmatic airways. Clinical & Experimental Allergy, 27: 389–395. doi: 10.1111/j.1365-2222.1997.tb00723.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 16 May 1996; revised 20 August 1996; accepted 27 August 1996.
- bronchial epithelial cells;
- mucosal immunity;
Background Asthma is characterized by alterations of the bronchial epithelium associated with inflammatory cell infiltrates and sub-epithelial fibrosis. Transforming Growth Factor-β (TGF-β) is an anti-inflammatory and fibrosing cytokine normally present in bronchial epithelial cells and also potentially produced by inflammatory cells. Thus, TGF-β could play a role in the asthmatic process, and its expression could be modified in asthmatic airways.
Objective To test this latter hypothesis, we studied the bronchial distribution of TGF-β in asthmatic patients.
Methods TGF-β1, 2, 3 distribution was studied by immunohistochemistry in bronchial biopsies from 12 asthmatic patients and 10 non-asthmatic subjects.
Results Bronchial epithelial cells from asthmatics were negative or faintly positive while a bright staining was detected in these from non-asthmatics (P < 0.0l). In both groups, when inflammatory cells were present beneath the basement membrane, they were stained by the anti-TGF-β antibody.
Conclusion This study shows an altered compartimentalization of TGF-β in asthma. (a) TGF-β is scarse in asthmatic bronchial epithelial cells, which could favour the perennization of the bronchial inflammation, and (b) TGF-β is present in inflammatory cells beneath the basement membrane, where it could be involved in the frequent sub-epithelial fibrosis.