An evaluation of peripheral blood eosinophil/basophil progenitors following nasal allergen challenge in patients with allergic rhinitis
Article first published online: 15 NOV 2004
Clinical & Experimental Allergy
Volume 35, Issue 1, pages 39–44, January 2005
How to Cite
Wilson, A. M., Duong, M., Crawford, L. and Denburg, J. (2005), An evaluation of peripheral blood eosinophil/basophil progenitors following nasal allergen challenge in patients with allergic rhinitis. Clinical & Experimental Allergy, 35: 39–44. doi: 10.1111/j.1365-2222.2004.02072.x
- Issue published online: 15 NOV 2004
- Article first published online: 15 NOV 2004
- Submitted 14 December 2003; revised 19 April 2004; accepted 20 May 2004
- eosinophil/basophil progenitors;
- induced sputum;
- nasal allergen challenge;
- nasal peak inspiratory flow;
- seasonal allergic rhinitis
Objective To evaluate the effect of a single nasal allergen challenge on peripheral blood eosinophil/basophil (Eo/B) progenitor cells and induced sputum eosinophil counts in subjects with allergic rhinitis.
Methods Sixteen adults entered a sequential nasal control and allergen challenge study, outside the pollen season. Blind assessment of peripheral blood Eo/B progenitor colony forming units (CFU), induced sputum and nasal lavage cell counts was made before and 24 h after both challenges. Subjects recorded their rhinitis symptoms and nasal peak inspiratory flow, hourly at home, following both challenges.
Results When comparing the values 24 h after the control vs. the allergen challenge, there were no significant differences in Eo/B progenitor CFU (control (mean, SD): 3.6 (1.0)/106 cells; allergen: 4.4 (1.1)/106 cells) or sputum eosinophils (control (median, inter-quartile range): 1.0 (0.3–1.7)%; allergen: 0.7 (0.0–1.3)%) despite a significant increase in the percentage (median (inter-quartile range) of eosinophils in nasal lavage (control: 0.6 (0.1–0.9)%; allergen; 1.9 (0.9–8.1)% and significant worsening of nasal peak inspiratory flow and rhinitis symptoms.
Conclusions Despite a significant increase in nasal symptoms and lavage eosinophil counts, a single nasal allergen challenge was not sufficient to elicit a measurable haemopoietic response in circulation, or an increase in sputum eosinophil counts.