Allergic airway inflammation induces tachykinin peptides expression in vagal sensory neurons innervating mouse airways

Authors


Q. Thai Dinh, Department of Internal Medicine and Clinical Research Unit of Allergy, Charité School of Medicine, Humboldt and Freie University Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
E-mail: q-thai.dinh@charite.de

Summary

Background Allergic airway inflammation has been shown to induce pro-inflammatory neuropeptides such as tachykinin peptides substance P (SP) and neurokinin A (NKA) together with related peptide like calcitonin gene-related peptide (CGRP) in nodose sensory neurons innervating guinea-pig airways.

Objective The present study was designed to examine the effects of allergen sensitization and challenge on the SP/NKA expression in the jugular–nodose ganglion neurons innervating the murine airways.

Methods Using retrograde neuronal tracing technique in combination with double-labelling immunohistochemistry, the expression of SP/NKA was investigated in a murine model of allergic airway inflammation.

Results Allergic airway inflammation was found to induce the expression of SP/NKA (13.2±1.43% vs. 5.8±0.37%, P<0.01) in large-diameter (>20 μm) vagal sensory neurons retrograde labelled with Fast blue dye from the main stem bronchi.

Conclusion Based on the induction of tachykinins in airway-specific large-sized jugular–nodose ganglia neurons by allergic airway inflammation, the present study suggests that allergen sensitization and challenge may lead to de novo induction of tachykinins in neurons. This may partly contribute to the pathogenesis of airways diseases such as allergic airway inflammation.

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