Cysteinyl leukotrienes induce monocyte chemoattractant protein 1 in human monocytes/macrophages
Article first published online: 14 SEP 2005
Clinical & Experimental Allergy
Volume 35, Issue 9, pages 1214–1219, September 2005
How to Cite
Ichiyama, T., Hasegawa, M., Ueno, Y., Makata, H., Matsubara, T. and Furukawa, S. (2005), Cysteinyl leukotrienes induce monocyte chemoattractant protein 1 in human monocytes/macrophages. Clinical & Experimental Allergy, 35: 1214–1219. doi: 10.1111/j.1365-2222.2005.02323.x
- Issue published online: 14 SEP 2005
- Article first published online: 14 SEP 2005
- Submitted 13 October 2004; revised 26 April 2005; accepted 7 July 2005
- cysteinyl leukotrienes;
- monocyte chemoattractant protein 1;
Background Monocytes/macrophages have a cysteinyl leukotriene 1 (CysLT1) receptor, but its function is poorly understood.
Objective To elucidate the biological function of the CysLT1 receptor of human monocytes/macrophages.
Methods We examined the production of TNF-α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, monocyte chemoattractant protein 1 (MCP-1), macrophage colony-stimulating factor (M-CSF), and eotaxin induced by CysLTs (leukotriene (LT)C4, -D4, and -E4) in THP-1 cells, a human monocytic leukaemia cell line, and peripheral blood CD14+ monocytes/macrophages. Moreover, we examined the effect of CysLTs on the expression of β-chemokine receptor 2B (CCR2B) as the receptor of MCP-1 by Western blot analysis.
Results ELISA revealed that CysLTs induced MCP-1 in THP-1 cells and peripheral blood CD14+ monocytes/macrophages, but not other cytokines. PCR demonstrated that CysLTs increased MCP-1 mRNA expression in THP-1 cells, and Western blotting showed that CysLTs increased the expression of CCR2B in THP-1 cells. Moreover, we demonstrated that pranlukast, a CysLT1 receptor antagonist, blocked MCP-1 production by CysLTs in THP-1 cells almost completely, and partially inhibited MCP-1 release by CysLTs in peripheral blood CD14+ monocytes/macrophages and CCR2B expression by CysLTs in THP-1 cells.
Conclusion CysLTs induce MCP-1 and increase CCR2B expression in human monocytes/macrophages.