Female mice are more susceptible to the development of allergic airway inflammation than male mice
Article first published online: 9 NOV 2005
Clinical & Experimental Allergy
Volume 35, Issue 11, pages 1496–1503, November 2005
How to Cite
Melgert, B. N., Postma, D. S., Kuipers, I., Geerlings, M., Luinge, M. A., van der Strate, B. W. A., Kerstjens, H. A. M., Timens, W. and Hylkema, M. N. (2005), Female mice are more susceptible to the development of allergic airway inflammation than male mice. Clinical & Experimental Allergy, 35: 1496–1503. doi: 10.1111/j.1365-2222.2005.02362.x
- Issue published online: 9 NOV 2005
- Article first published online: 9 NOV 2005
- Submitted 25 February 2005; revised 15 July 2005; accepted 8 August 2005
- airway hyper-responsiveness;
- regulatory T cells;
- sex hormones
Background In humans the prevalence of asthma is higher among females than among males after puberty. The reason for this phenomenon is not clear.
Objective We tested the hypothesis that female mice are more susceptible to the development of allergic asthma than male mice and studied allergic immune responses in the lung.
Methods We compared allergic airway inflammation, i.e. methacholine (MCh) responsiveness, serum IgE, and cytokines, and the number of the different leucocytes in lungs of male and female BALB/c mice, twice-sensitized to ovalbumin (OVA) and subsequently challenged with OVA (OVA-mice) or phosphate-buffered saline (PBS-mice) aerosols on days 24–26, 30, and 31.
Results OVA challenge significantly increased MCh responsiveness, numbers of eosinophils, CD4+ T cells, CD4+/CD25+ T cells, B cells, and levels of Thelper (Th)2 cytokines, total, and OVA-specific IgE. There was, however, also an effect of gender, with female mice responding to OVA challenges with higher numbers of eosinophils, CD4+ T cells, B cells, and levels of IL-4, IL-13, IFN-γ, total, and OVA-specific IgE than male mice. In contrast, female PBS-mice had significantly lower percentages of regulatory CD4+/CD25+ T cells than males (females 4.2±0.2% vs. males 5.3±0.1% of CD4+ T cells, P<0.05).
Conclusion Female mice develop a more pronounced type of allergic airway inflammation than male mice after OVA challenge. The reduced percentage of regulatory T cells in the lungs of female PBS-mice may indicate that the level of these cells in the lung during the sensitization phase is important for the development and/or progression of an allergic immune response after multiple OVA challenges.