Exhaled breath condensate levels of eotaxin and macrophage-derived chemokine in stable adult asthma patients
Article first published online: 22 DEC 2005
Clinical & Experimental Allergy
Volume 36, Issue 1, pages 44–51, January 2006
How to Cite
Ko, F. W. S., Lau, C. Y. K., Leung, T. F., Wong, G. W. K., Lam, C. W. K., Lai, C. K. W. and Hui, D. S. C. (2006), Exhaled breath condensate levels of eotaxin and macrophage-derived chemokine in stable adult asthma patients. Clinical & Experimental Allergy, 36: 44–51. doi: 10.1111/j.1365-2222.2006.02398.x
- Issue published online: 22 DEC 2005
- Article first published online: 22 DEC 2005
- Submitted 16 May 2005; revised 9 September 2005; accepted 12 October 2005
- exhaled breath condensate;
- macrophage-derived chemokine
Background Asthma is associated with esoinophilic airway inflammation and overproduction of T-helper type 2 (Th2) lymphocyte-related cytokines.
Objective This study assessed the eosinophil chemoattractant eotaxin and Th2-specific macrophage-derived chemokine (MDC) in the adult asthmatic airway. Eotaxin and MDC levels were determined in exhaled breath condensate (EBC) obtained from adult patients with asthma.
Methods Fifty-four asthmatics (20 male, mean (SD) age 40 (12) years and percentage predicted forced expiratory volume in 1 s (FEV1) 81.7 (20.8)) and 20 age- and sex-matched controls were studied. EBC was collected using EcoScreen by 10 min of tidal breathing with a nose clip. Concentrations of eotaxin and MDC were measured by ELISA.
Results Asthma patients on inhaled corticosteroid (ICS) had a higher median interquartile range (IQR) level of eotaxin than the steroid-naïve asthmatics (18.5 (17.7–20.1) vs. 17.9 (17.0–18.6) pg/mL, P=0.02) and controls (18.5 (17.7–20.1) pg/mL vs 17.4 (16.3–18.0) pg/mL, P=0.001). Eotaxin level in EBC had a significant negative correlation with the FEV1/forced vital capacity ratio (r=−0.43, P=0.03) in steroid-naïve asthmatics. EBC MDC level was higher in subjects on ICS than the steroid naïve asthmatics (120 (118–125) vs. 117 (116–119) pg/mL, P=0.01) and the controls (120 (118–125) vs. 117 (116–120) pg/mL, P=0.02).
Conclusions Eotaxin and MDC could be measured in EBC of adults with asthma. EBC eotaxin and MDC levels were higher in asthmatics on ICS than the steroid-naïve asthmatics or controls. Exhaled chemokines may be potential non-invasive markers for assessing airway inflammation in asthmatics.