Surfactant protein D inhibits early airway response in Aspergillus fumigatus-sensitized mice

  1. V. J. Erpenbeck1,2,
  2. M. Ziegert1,
  3. D. Cavalet-Blanco3,
  4. C. Martin4,
  5. R. Baelder1,
  6. T. Glaab2,
  7. A. Braun1,
  8. W. Steinhilber5,
  9. B. Luettig1,
  10. S. Uhlig4,6,
  11. H. G. Hoymann1,
  12. N. Krug1,
  13. J. M. Hohlfeld1,2

Article first published online: 27 JUN 2006

DOI: 10.1111/j.1365-2222.2006.02524.x

Issue

Clinical & Experimental Allergy

Clinical & Experimental Allergy

Volume 36, Issue 7, pages 930–940, July 2006

Additional Information

How to Cite

Erpenbeck, V. J., Ziegert, M., Cavalet-Blanco, D., Martin, C., Baelder, R., Glaab, T., Braun, A., Steinhilber, W., Luettig, B., Uhlig, S., Hoymann, H. G., Krug, N. and Hohlfeld, J. M. (2006), Surfactant protein D inhibits early airway response in Aspergillus fumigatus-sensitized mice. Clinical & Experimental Allergy, 36: 930–940. doi: 10.1111/j.1365-2222.2006.02524.x

Author Information

  1. 1

    Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany,

  2. 2

    Department of Respiratory Medicine, Hannover Medical School, Hannover, Germany,

  3. 3

    Hospital Sao Lucas da Pontificia Universidade Catolica do Rio Grande do Sul, Porto Alegre, Brazil,

  4. 4

    Division of Pulmonary Pharmacology, Research Center Borstel, Borstel, Germany,

  5. 5

    ALTANA Pharma AG, Konstanz, Germany

  6. 6

    Institute of Pharmacology and Toxicology, RWTH Aachen, Aachen, Germany

*Correspondence: Jens M. Hohlfeld, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs-Straβe 1, 30625 Hannover, Germany. E-mail: hohlfeld@item.fraunhofer.de

Publication History

  1. Issue published online: 27 JUN 2006
  2. Article first published online: 27 JUN 2006
  3. Submitted 15 July 2004; revised 18 April 2006; accepted 29 April 2006

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Keywords:

  • allergic inflammation;
  • asthma;
  • collectins;
  • lung function;
  • pulmonary surfactant

Summary

Background The surfactant protein SP-D has been reported to reduce bronchial hyper-responsiveness, blood eosinophilia, and T-helper type 2 cytokines in models of allergic asthma. However, little is known about the functional effect of SP-D on the early airway response upon allergen inhalation, which is an important feature of this disease.

Objective We investigated whether SP-D is able to reduce the immediate allergen-induced mediator release and the early bronchial obstruction in addition to its effects on airway inflammation and bronchial hyperresponsiveness in an Aspergillus fumigatus mouse asthma model.

Methods A. fumigatus-sensitized mice were treated with a recombinant fragment of human SP-D or placebo. Lung functions were measured in orotracheally intubated, spontaneously breathing animals using body plethysmography. In addition, passively sensitized precision-cut lung slices (PCLS) were used to determine the effect of SP-D on allergen-induced histamine release.

Results SP-D inhibited the allergen-induced early airway response and reduced airway hyperresponsiveness compared with placebo. Eosinophilia in bronchoalveolar lavage and lung tissue was reduced after SP-D treatment, possibly by reducing eotaxin levels in the lung. Furthermore, SP-D treatment reduced the allergen-induced histamine release from PCLS.

Conclusions These data suggest that SP-D not only reduces allergen-induced eosinophilic inflammation and airway hyperresponsiveness but also provides protection against early airway obstruction by inhibition of early mediator release.

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