Allergen concentration in natural rubber latex
Article first published online: 4 JUL 2006
Clinical & Experimental Allergy
Volume 36, Issue 8, pages 1078–1086, August 2006
How to Cite
Yeang, H.-Y., Hamilton, R. G., Bernstein, D. I., Arif, S. A. M., Chow, K. .-S., Loke, Y. .-H., Raulf-Heimsoth, M., Wagner, S., Breiteneder, H. and Biagini, R. E. (2006), Allergen concentration in natural rubber latex. Clinical & Experimental Allergy, 36: 1078–1086. doi: 10.1111/j.1365-2222.2006.02531.x
- Issue published online: 4 JUL 2006
- Article first published online: 4 JUL 2006
- Submitted 25 January 2006; revised 27 March 2006; accepted 18 May 2006
Vol. 40, Issue 5, 831, Article first published online: 19 APR 2010
- Hevea brasiliensis;
- latex allergy;
- natural rubber serology;
- skin prick test
Background Hevea brasiliensis latex serum is commonly used as the in vivo and in vitro reference antigen for latex allergy diagnosis as it contains the full complement of latex allergens.
Objective This study quantifies the concentrations of the significant allergens in latex serum and examines its suitability as an antigen source in latex allergy diagnosis and immunotherapy.
Methods The serum phase was extracted from centrifuged latex that was repeatedly freeze-thawed or glycerinated. Quantitation of latex allergens was performed by two-site immunoenzymetric assays. The abundance of RNA transcripts of the latex allergens was estimated from the number of their clones in an Expressed Sequence Tags library.
Results The latex allergens, Hev b 1, 2, 3, 4, 5, 6, 7 and 13, were detected in freeze-thawed and glycerinated latex serum at levels ranging from 75 (Hev b 6) to 0.06 nmol/mg total proteins (Hev b 4). Hev b 6 content in the latex was up to a thousand times higher than the other seven latex allergens, depending on source and/or preparation procedure. Allergen concentration was reflected in the abundance of mRNA transcripts. When used as the antigen, latex serum may bias the outcome of latex allergy diagnostic tests towards sensitization to Hev b 6. Tests that make use of latex serum may fail to detect latex-specific IgE reactivity in subjects who are sensitized only to allergens that are present at low concentrations.
Conclusion Latex allergy diagnostics and immunotherapy that use whole latex serum as the antigen source may not be optimal because of the marked imbalance of its constituent allergens.