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Association of neuropeptides with Th1/Th2 balance and allergic sensitization in children

Authors


  • LISAplus Study Group: GSF-National Research Centre for Environment and Health, Neuherberg, Institute of Epidemiology (H. E. Wichmann, J. Heinrich, T. Illig); IUF-Institut für Umweltmedizinische Forschung Düsseldorf, Epidemiology and Particle Research Group (U. Krämer, U. Ranft, R. P. F. Schins); UFZ-Centre for Environmental Research, Leipzig, Department of Environmental Immunology (I. Lehmann, G. Herberth), Department of Human Exposure Research and Epidemiology (O. Herbarth, S. Röder, U. Rolle-Kampzcyk, U. Schlink, M. Rehwagen); Marien-Hospital Wesel, Department of Pediatrics (A. von Berg, B. Schaaf, C. Scholten, C. Bollrath); Technical University Munich, Department of Pediatrics (C-P. Bauer), Department of Dermatology and Allergy (J. Ring), ZAUM–Center for Allergy and Environment (H. Behrendt); Municipal Hospital ‘St Georg’, Leipzig, Children's Hospital (M. Borte, U. Diez); Ludwig-Maximillian-University Munich, Dr von Haunersches Kinderspital, Pediatrical Immunology (S. Krauss-Etschmann); Medical University Lübeck, Department of Social Medicine (T. Schäfer).

Correspondence:
Gunda Herberth, UFZ Centre for Environmental Research Leipzig-Halle, Department of Environmental Immunology, Permoserstrasse 15, Leipzig 04318, Germany.
E-mail: gunda.herberth@ufz.de

Summary

Background Among neurogenic factors, the neuropeptides have an important regulatory influence on immune system activity and may lead to allergic sensitization.

Objective The aim of our study was to investigate the relationship of the neuropeptides vasoactive intestinal peptide (VIP), somatostatin (SOM) and substance P (SP) on modulation of Th1/Th2 balance and allergic sensitization in children.

Methods Within the LISAplus (Life style–Immune system–Allergy) study, blood samples of 321 six-year-old children were analysed for concentration of neuropeptides, Th1 and Th2 cytokines, transcription factors for T cell regulation and suppressors of cytokine signalling. In addition, samples were screened for specific IgE against inhalant and food allergens.

Results Children with high SOM values showed a Th2 polarization and a reduced expression of FOXP3, the marker for regulatory T cells. High (VIP) levels correlated inversely with the expression of T cell transcription factors (Tbet and SOCS3). In contrast, elevated levels of SP were associated with reduced GATA3 and SOCS3 expression and with increased IFN-γ concentrations. Allergic sensitization was more prevalent in children with higher SOM and VIP concentrations but not associated with SP levels.

Conclusion Our data reveal an association between neuropeptides and modulatory effects on immune cells in vivo, especially on Th1/Th2 balance with a correlation to allergic sensitization in children. We suggest that elevated SOM and VIP concentrations and the inducing factors should be considered as allergy risk factors.

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