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Clinical & Experimental Allergy

Roles of the transmembrane domain and the cytoplasmic domain of FcɛRIα in immunoglobulin E-mediated up-regulation of surface FcɛRI expression

Authors

  • Y. Okayama,

    1. Research Unit for Allergy Transcriptome, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan,
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  • S. Kagaya,

    1. Research Unit for Allergy Transcriptome, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan,
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  • K. Yuki,

    1. Research Unit for Allergy Transcriptome, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan,
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  • T. Arai,

    1. Laboratory of Seeds Finding Technology, Eisai Co. Ltd., Tsukuba, Japan
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  • H. Saito

    1. Research Unit for Allergy Transcriptome, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan,
    2. Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan
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  • 1Y. O. and S. K. contributed equally to this work.

Correspondence:
Yoshimichi Okayama, Research Unit for Allergy Transcriptome, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
E-mail: yokayama@rcai.riken.go.jp

Summary

Background Human mast cells express both FcɛRIα and FcγRIα. IgE up-regulates FcɛRIα expression, but IgG1 does not up-regulate FcγRIα expression. The transmembrane domain (TM) of FcγRIα determines the stability of cell surface expression of this receptor.

Objective The aim of this study was to clarify the roles of the TM and cytoplasmic domain (CY) of FcɛRIα in IgE-mediated FcɛRI up-regulation.

Methods Chimeric receptors created by domain shuffling between FcɛRIα and FcγRIα were transduced into human mast cell line HMC-1. Cell surface expression of the chimeric receptors and the effect of IgE or IgG1 on chimeric receptor expression were examined by FACS. The association of the chimeric receptors with FcRγ was investigated by immunoprecipitation.

Results The results showed that the TM and CY of FcɛRIα are not essential for IgE-mediated up-regulation of surface FcɛRI.

Conclusion The extracellular domain of each Fc receptor determines the diversity of Ig-regulated Fc receptor expression.

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