Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j 1 and Par j 2 allergens
Article first published online: 23 JAN 2007
Clinical & Experimental Allergy
Volume 37, Issue 2, pages 243–250, February 2007
How to Cite
González-Rioja, R., Ferrer, A., Arilla, M.C., Ibarrola, I., Viguera, A.R., Andreu, C., Martínez, A. and Asturias, J.A. (2007), Diagnosis of Parietaria judaica pollen allergy using natural and recombinant Par j 1 and Par j 2 allergens. Clinical & Experimental Allergy, 37: 243–250. doi: 10.1111/j.1365-2222.2007.02643.x
- Issue published online: 23 JAN 2007
- Article first published online: 23 JAN 2007
- Submitted 27 April 2006; revised 25 September 2006; accepted 30 Oct 2006
- circular dichroism;
- IgE binding;
- Par j 1;
- Par j 2;
- Parietaria judaica;
- recombinant allergen;
- skin prick test
Background Parietaria judaica pollen is one of the main causes of allergic diseases in the Mediterranean area and contains two major allergens, called Par j 1 and Par j 2.
Objective To evaluate the diagnostic potential of natural and recombinant forms of Par j 1 and Par j 2 in comparison with standardized P. judaica pollen extract.
Methods Thirty patients allergic to P. judaica pollen and 15 control patients were investigated. Skin prick tests and determination of specific IgE levels were performed with commercial P. judaica extract, natural Par j 1 and Par j 2, and recombinant forms of both allergens expressed in P. pastoris.
Results The whole group of patients with allergy to P. judaica had a positive skin test reaction to purified nPar j 1–Par j 2 and rPar j 2 at 5 μg/mL, and no false-positive reactions were detected. Natural and recombinant Par j 1 and Par j 2 showed no significantly different responses in skin tests compared with P. judaica extract. A high correlation was found between the serum-specific IgE levels to P. judaica extract vs. natural (R=0.996; P<0.001) and recombinant allergens (R=0.887 and 0.982 for rPar j 1 and rPar j 2, respectively; P<0.001). rPar j 2 displayed a 100% sensitivity and specificity among P. judaica-allergic patients.
Conclusions In vivo and in vitro diagnosis of P. judaica pollen allergy could be simplified using rPar j 2. This protein showed comparable IgE response and skin prick reactivity with those produced by P. judaica pollen extract.