Inhibitory effects of N-acetylcysteine on the functional responses of human eosinophils in vitro
Article first published online: 20 APR 2007
Clinical & Experimental Allergy
Volume 37, Issue 5, pages 714–722, May 2007
How to Cite
Martinez-Losa, M., Cortijo, J., Juan, G., O'Connor, J. E., Sanz, M. J., Santangelo, F. and Morcillo, E. J. (2007), Inhibitory effects of N-acetylcysteine on the functional responses of human eosinophils in vitro. Clinical & Experimental Allergy, 37: 714–722. doi: 10.1111/j.1365-2222.2007.02694.x
- Issue published online: 20 APR 2007
- Article first published online: 20 APR 2007
- Submitted 24 May 2006; revised 17 December 2006; accepted 9 February 2007
- eosinophil cationic protein;
- human eosinophils;
- reactive oxygen species
Background Oxidative stress appears to be relevant in the pathogenesis of inflammation in allergic diseases like bronchial asthma. Eosinophils are oxidant-sensitive cells considered as key effectors in allergic inflammation.
Objective The aim of this work was to study the effects of the clinically used antioxidant N-acetyl-l-cysteine (NAC) on the functional responses of human-isolated eosinophils.
Methods Human eosinophils were purified from the blood of healthy donors by a magnetic bead separation system. The effects of NAC were investigated on the generation of reactive oxygen species (chemiluminescence and flow cytometry), Ca2+ signal (fluorimetry), intracellular glutathione (GSH; flow cytometry), p47phox–p67phox translocation (Western blot) and eosinophil cationic protein (ECP) release (radioimmunoassay).
Results NAC (0.1–1 mm) inhibited the extracellular generation of oxygen species induced by N-formyl-l-methionyl-l-leucyl-l-phenylalanine (fMLP) and eotaxin (in the presence of IL-5) with −logIC50 values of 3.61±0.03 and 3.36±0.09, respectively. Also, the intracellular generation of hydrogen peroxide was virtually abolished by NAC (0.5–1 mm). NAC (1 mm) did not alter the fMLP-induced Ca2+ signal but augmented the eosinophil content of reduced GSH and inhibited p47phox–p67phox translocation. NAC inhibited the release of ECP (∼90% inhibition at 1 mm) from fMLP-activated eosinophils.
Conclusion Inhibition by NAC of human eosinophil functions in vitro is potentially useful in the treatment of allergic inflammation.