Lipoxin A4 levels in asthma: relation with disease severity and aspirin sensitivity
Article first published online: 14 AUG 2007
Clinical & Experimental Allergy
Volume 37, Issue 10, pages 1494–1501, October 2007
How to Cite
Çelik, G. E., Erkekol, F. O., Mιsιrlιgil, Z. and Melli, M. (2007), Lipoxin A4 levels in asthma: relation with disease severity and aspirin sensitivity. Clinical & Experimental Allergy, 37: 1494–1501. doi: 10.1111/j.1365-2222.2007.02806.x
- Issue published online: 14 AUG 2007
- Article first published online: 14 AUG 2007
- Submitted 20 March 2007; revised 4 June 2007; accepted 4 July 2007
- aspirin-exacerbated respiratory disease;
- 15-epi-lipoxin A4;
- lipoxin A4;
Background Lipoxin (LX) A4, an endogenous anti-inflammatory eicosanoid, has been found to be low in patients with severe asthma. However, few studies also suggested more diminished LX A4 levels in aspirin-exacerbated respiratory disease (AERD) when compared with aspirin-tolerant asthma (ATA). It is, therefore, currently not clear whether the asthma severity or the presence of AERD has a primary role in the disturbed LX metabolism.
Objective To detect LX A4 and 15-epi-LX A4 levels in asthma patients with and without AERD of comparable severity.
Methods The study groups consisted of 22 subjects with AERD, 22 subjects with ATA and 10 volunteers without asthma and aspirin sensitivity. Whole-blood samples were stimulated with calcium ionophore, A23187 (5 × 10−5 m) and A23187 (5 × 10−5 m)+aspirin (10−4 m). LX A4 and 15-epi-LX A4 levels were analysed by the enzyme immune assay method.
Results Severe asthma patients in both AERD [0.5 (0.8)] ng/mL and ATA [0.5 (0.45) ng/mL] groups showed diminished generation for LX A4 to stimulation with A23187 in comparison with other severity degrees in their groups (P=0.02 and 0.046, respectively). LX A4 generation in both severe groups was comparable with each other (P>0.05). Although severe cases with AERD showed a diminished capacity to generate 15-epi-LX A4, this did not reach statistical significance.
Conclusion This study indicated that diminished LX A4 generation was unique to severe asthma phenotype regardless of comorbid aspirin sensitivity.
Clinical Implications Lower LX A4 levels in severe asthma would suggest a possibility for LX analogues as future treatment options in these patients.