Latex allergy: low prevalence of immunoglobulin E to highly purified proteins Hev b 2 and Hev b 13
Article first published online: 10 SEP 2007
Clinical & Experimental Allergy
Volume 37, Issue 10, pages 1502–1511, October 2007
How to Cite
Palosuo, T., Lehto, M., Kotovuori, A., Kalkkinen, N., Blanco, C., Poza, P., Carrillo, T., Hamilton, R. G., Alenius, H., Reunala, T. and Turjanmaa, K. (2007), Latex allergy: low prevalence of immunoglobulin E to highly purified proteins Hev b 2 and Hev b 13. Clinical & Experimental Allergy, 37: 1502–1511. doi: 10.1111/j.1365-2222.2007.02810.x
- Issue published online: 10 SEP 2007
- Article first published online: 10 SEP 2007
- Submitted 21 December 2006; revised 26 May 2007; accepted 29 June 2007
- Hev b 2;
- Hev b 13;
- IgE antibodies;
- latex hypersensitivity;
Background Hevea brasiliensis (Hev b) 2 and Hev b 13 have recently been identified as major latex allergens by detecting specific IgE antibodies in >50% of sera from Hev b latex-allergic individuals.
Objective We assessed the prevalence rates for sensitization to extensively purified latex allergens in patients from three diverse geographical areas.
Methods Native Hev b 2, Hev b 5, Hev b 6.01 and Hev b 13 were purified by non-denaturating chromatography and were used in ELISAs to assess sera from 215 latex-allergic patients and 172 atopic non-sensitized controls from Finland, Spain and the United States to detect allergen-specific IgE antibodies.
Results Unexpectedly, even highly purified Hev b 13 contained epitope(s) to which Hev b 6-specific human IgE antibodies bound effectively. Further purification, however, reduced the prevalence of IgE antibody reactivity to low levels: 15%, 5% and 11% for Hev b 2, and 18%, 30% and 27% for Hev b 13 among latex-allergic Finnish, Spanish and American patients, respectively. Interestingly, Finnish patients had a lower prevalence of Hev b 5-specific IgE antibody (28%) as compared with Spanish (49%) and American (71%) patients. The prevalence of Hev b 6.01-specific IgE reactivity was uniformly >50% in all three populations.
Conclusion Neither Hev b 2 nor Hev b 13 appear to be major latex allergens when evaluated in serological assays using highly purified allergens. The reason(s) for the observed differences in published sensitization rates in various geographic regions requires further study. The purity of the allergen preparations has a marked impact on the accuracy of latex-specific IgE antibody detection in epidemiological studies and in the serological diagnosis of latex allergy.