Human γδ T cells modulate the mite allergen-specific T-helper type 2-skewed immunity
Article first published online: 19 SEP 2007
Clinical & Experimental Allergy
Volume 37, Issue 11, pages 1681–1687, November 2007
How to Cite
Korematsu, S., Tanaka, Y., Nagakura, T., Minato, N. and Izumi, T. (2007), Human γδ T cells modulate the mite allergen-specific T-helper type 2-skewed immunity. Clinical & Experimental Allergy, 37: 1681–1687. doi: 10.1111/j.1365-2222.2007.02826.x
- Issue published online: 19 SEP 2007
- Article first published online: 19 SEP 2007
- Submitted 18 August 2006; revised 13 August 2007; accepted 14 August 2007
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Background γδ T cells have been described as one of immune regulators in patients with infection, malignancy, and allergy.
Objective To elucidate the ability of γδ T cells as an allergen immunotherapy candidate, the effectiveness of human γδ T cells in allergen-specific T-helper type 2 (Th2)-type T cells was evaluated in vitro.
Methods House dust mite-specific Th2-type T cell clones, Bacillus Calmette–Guerin (BCG)-specific Th1-type T cell clones, and γδ T cell lines were established from the peripheral blood mononuclear cells of two patients with allergic rhinitis. The effectiveness of γδ T cells and BCG-specific Th1-type T cell clones in the modulation of allergen-specific Th2 cells in terms of their cytokine productions was evaluated.
Results In response to cognate antigens, the γδ T cell lines demonstrated a proliferation and production of IFN-γ that exceeded that of BCG-specific Th1-type T cell clones (mean stimulation index: 14.5 vs. 2.8, mean IFN-γ: 130.5 vs. 10.0 pg/mL). When the γδ T cell lines and mite-allergen-specific Th2 clones were co-cultured with each other, only the levels of IL-4 (mean, −87%) decreased, but not the levels of IL-5 and IL-13, with an increasing concentration of γδ T cell antigen and IFN-γ production (mean, +730%).
Conclusion These results demonstrated that γδ T cells derived from allergic patients might thus have a partial ability to modulate allergen-specific Th2-skewed immunity.