Background γδ T cells have been described as one of immune regulators in patients with infection, malignancy, and allergy.
Objective To elucidate the ability of γδ T cells as an allergen immunotherapy candidate, the effectiveness of human γδ T cells in allergen-specific T-helper type 2 (Th2)-type T cells was evaluated in vitro.
Methods House dust mite-specific Th2-type T cell clones, Bacillus Calmette–Guerin (BCG)-specific Th1-type T cell clones, and γδ T cell lines were established from the peripheral blood mononuclear cells of two patients with allergic rhinitis. The effectiveness of γδ T cells and BCG-specific Th1-type T cell clones in the modulation of allergen-specific Th2 cells in terms of their cytokine productions was evaluated.
Results In response to cognate antigens, the γδ T cell lines demonstrated a proliferation and production of IFN-γ that exceeded that of BCG-specific Th1-type T cell clones (mean stimulation index: 14.5 vs. 2.8, mean IFN-γ: 130.5 vs. 10.0 pg/mL). When the γδ T cell lines and mite-allergen-specific Th2 clones were co-cultured with each other, only the levels of IL-4 (mean, −87%) decreased, but not the levels of IL-5 and IL-13, with an increasing concentration of γδ T cell antigen and IFN-γ production (mean, +730%).
Conclusion These results demonstrated that γδ T cells derived from allergic patients might thus have a partial ability to modulate allergen-specific Th2-skewed immunity.
If you can't find a tool you're looking for, please click the link at the top of the page to "Go to old article view". Alternatively, view our Knowledge Base articles for additional help. Your feedback is important to us, so please let us know if you have comments or ideas for improvement.