1These authors contributed equally to this work.
Trigeminal nasal-specific neurons respond to nerve growth factor with substance-P biosynthesis
Article first published online: 26 FEB 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 38, Issue 7, pages 1203–1211, July 2008
How to Cite
Mingomataj, E., Dinh, Q. T., Groneberg, D., Feleszko, W., Schmeck, B., Joachim, R., Noga, O., Nagel, S., Klapp, B. F. and Fischer, A. (2008), Trigeminal nasal-specific neurons respond to nerve growth factor with substance-P biosynthesis. Clinical & Experimental Allergy, 38: 1203–1211. doi: 10.1111/j.1365-2222.2008.02957.x
- Issue published online: 8 JUL 2008
- Article first published online: 26 FEB 2008
- Submitted 6 January 2006; revised 27 November 2007; accepted 6 December 2007
- glutamate and neurofilament protein;
- nasal mucosa;
- nerve growth factor;
- neuronal tracing;
- trigeminal ganglion neuron;
- upper airway nerve
Background Nerve growth factor (NGF) has been found to induce substance-P biosynthesis in large-diameter A-fibres vagal airway neurons. However, the effect of NGF on trigeminal neurons innervating the nasal mucosa of the mouse has not been investigated so far.
Objective NGF has been implicated in allergic diseases by modulating sensory nerves. Therefore, the present study investigated the effect of NGF on neuropeptides expression such as substance-P and glutamate in nasal trigeminal neurons.
Methods Using neuronal tracing in combination with double labelling immunohistochemistry the expression of substance-P, glutamate and neurofilament protein 68-kDa expression was examined in nasal-specific trigeminal neurons of BALB/c-mice.
Results The numbers of Fast blue-labelled trigeminal neurons expressing substance-P were significantly increased after NGF exposure (NGF-treated ganglia: 16.4 ± 0.6% vs. control: 7.0 ± 0.4%, P0.001). NGF treatment-induced substance-P biosynthesis in neurofilament-positive (NGF-treated ganglia: 8.6 ± 0.2% vs. control: 1.1 ± 0.2%, P0.001) as well as neurofilament-negative (NGF-treated ganglia: 7.8 ± 0.6% vs. control: 5.9 ± 0.4%, P=0.05) and non-glutamatergic neurons (NGF-treated ganglia: 11.8 ± 1.9% vs. control 1.1 ± 1.0%, P0.001) 24 h after NGF exposure.
Conclusion Under normal conditions, substance-P was expressed in nasal-specific neurofilament-negative, glutamatergic and C-fibre neurons. Nasal-specific trigeminal neurons respond to NGF treatment with substance-P biosynthesis in non-glutamatergic, neurofilament-positive as well as -negative neurons. These findings suggest that nasal-specific trigeminal neurons are composed of heterogenous subpopulations in relation to their peptide profiles and therefore may have different functions in neurogenic airway inflammation.