1These authors contributed equally
Allergen specific responses in cord and adult blood are differentially modulated in the presence of endotoxins
Article first published online: 3 SEP 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 38, Issue 10, pages 1627–1634, October 2008
How to Cite
Eiwegger, T., Mayer, E., Brix, S., Schabussova, I., Dehlink, E., Bohle, B., Barkholt, V. and Szépfalusi, Z. (2008), Allergen specific responses in cord and adult blood are differentially modulated in the presence of endotoxins. Clinical & Experimental Allergy, 38: 1627–1634. doi: 10.1111/j.1365-2222.2008.03080.x
- Issue published online: 9 OCT 2008
- Article first published online: 3 SEP 2008
- Submitted 21 November 2007; revised 11 April 2008, 3 June 2008; accepted 4 June 2008
- cord blood;
- endotoxin contamination;
- food allergens;
- toll like receptors
Background Endotoxins are common contaminants in allergen preparations and affect antigen-specific cellular responses. Distinct effects of endotoxin on cells in human umbilical cord and adult blood are poorly defined.
Objectives To examine the effect of endotoxins in allergen preparations on cellular responses in human cord and peripheral blood (PB).
Methods The endotoxin content in β lactoglobulin (BLG), the peanut allergen Ara h 1 and the major birch pollen allergen Bet v 1 was assessed. Proliferation and cytokine response of mononuclear cells towards contaminated and lipopolysaccharide (LPS)-free allergens were evaluated at different time-points. Fractions of contaminated BLG were generated and assayed on their immuno-stimulatory capacity. The involvement of toll-like receptor (TLR) 2 and 4 was investigated by blocking antibodies and TLR-transfected human embryonic kidney cells.
Results The proliferative response of cord blood (CB)-derived mononuclear cells towards allergen-preparations at day 3 was related to the level of LPS contamination. At day 7, proliferation was also detected in the absence of endotoxin. Cytokine production in CB was strongly affected by the content of endotoxin, TLR-4 dependent and not related to the allergen content. Allergen- and endotoxin-induced proliferative responses were generally significantly higher in CB than in adult blood.
Conclusion Endotoxins in allergen preparations confound allergen-specific cellular responses. The impact of these contaminations varies with the blood source (CB vs. PB), the type of allergen and is time- and dose-dependent.